Insertion junction: CLIP2.004641_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.004641
Chromosome:chromosome 6
Location:5252089
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g284200 LHCBM9 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGTCGGCACGTTGCCCTTAATCCTGGGTGCAAGGCCAAGGTTGCGAT
Internal bar code:ATCGTCCCGCCATCGCATCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2107
LEAP-Seq percent confirming:82.6087
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCAGCTCGGGAGTAACAA
Suggested primer 2:CTGGTCCACTCACAACTCCC