Insertion junction: CLIP2.004641_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.004641
Chromosome:chromosome 6
Location:5252094
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g284200 LHCBM9 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTCGCGTTCTTTCCGGAGACCTGGCATCGAGAACCCGAGGCAATCACA
Internal bar code:AAGTCGTGGGTTGGGGATGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1887
LEAP-Seq percent confirming:44.8276
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGTCCACTCACAACTCCC
Suggested primer 2:CAGCAGCTCGGGAGTAACAA