| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004669 |
| Chromosome: | chromosome 12 |
| Location: | 7720943 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554850 | TRXH2,TRX7,TRXh2,TRXh2a | (1 of 9) K03671 - thioredoxin 1 (trxA); Thioredoxin h2, cytosolic | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCTATCATCGCTTATACCGCTGTCACATTTCCATACTGTGTAGTCAA |
| Internal bar code: | AGGGGTGGACTTTCTACGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1016 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCTAAGTTTGCGACGCT |
| Suggested primer 2: | GCCCACTTTTCCCTTGCAAG |