Insertion junction: CLIP2.004835_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.004835
Chromosome:chromosome 16
Location:1282351
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g651400 (1 of 37) PF13639 - Ring finger domain (zf-RING_2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGCGGCTGTCCTACGCCTACGACGCCGTGGCACCCAGCTGCTGGGCTG
Internal bar code:ATCTATTCAAACATACTGTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2519
LEAP-Seq percent confirming:78.5714
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGTGCGGTTGAAGGAGAT
Suggested primer 2:CTCCCTTTGCCCCAAATTGC