| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.004837 |
| Chromosome: | chromosome 5 |
| Location: | 3194315 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232200 | NDA3,LCI18 | Mitochondrial NADH dehydrogenase; (1 of 1) PTHR22912:SF180 - 3-PHENYLPROPIONATE/CINNAMIC ACID DIOXYGENASE FERREDOXIN--NAD(+) REDUCTASE COMPONENT | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTACGTGTCCGTGTCAATGACCTGCACGCAGCCAGCACACACCAAAAAC |
| Internal bar code: | CGGGTAAACGTCTGGCGGCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1642 |
| LEAP-Seq percent confirming: | 97.3684 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACGCGCGTTTCTAATCCC |
| Suggested primer 2: | ACCACAGCCTTGTCGTACTG |