| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.004875 |
| Chromosome: | chromosome 1 |
| Location: | 6738921 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048150 | HLA8 | (1 of 2) 3.1.31.1 - Micrococcal nuclease / Micrococcal endonuclease; Transcriptional co-activator/nuclease induced by high light | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCTGCCCGTGTACGGGCGTACGGCGCTGCACGTACCGCAGGCAGTAC |
| Internal bar code: | TCACCGGCCAAGTAGCTGTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2639 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCAGATCAATGCGCACG |
| Suggested primer 2: | GTGGATGGGTGCAGCATAGT |