| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.004896 |
| Chromosome: | chromosome 3 |
| Location: | 3939979 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171179 | (1 of 226) IPR012337 - Ribonuclease H-like domain | CDS | |
| lncRNA_TCONS_00066652 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTCTGGGCTGCTGGGGCAGCAGCTCGCGGGGGTCGCACTCGCGGCCCC |
| Internal bar code: | CAGTGTTCTTGGGCGCCAAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 22.2222 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTTTGGATGGGGAAGGG |
| Suggested primer 2: | TGCTGCGGTCTGTTCTTGAT |