| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004920 |
| Chromosome: | chromosome 3 |
| Location: | 8363337 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g198950 | CGL30 | (1 of 3) PTHR31407//PTHR31407:SF18 - FAMILY NOT NAMED // PSBP DOMAIN-CONTAINING PROTEIN 4, CHLOROPLASTIC; Conserved in the Green Lineage | 3'UTR |
| Cre03.g800397 | (1 of 1) K18735 - protein SMG9 (SMG9) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGGATTGGGGGTTTGAGAGATTCCACGTGTGTGCGTTTCGTGGCTGCA |
| Internal bar code: | GCCGATATTCTGGTTGATATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2279 |
| LEAP-Seq percent confirming: | 71.7391 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGTGTGCTCTTGCGATT |
| Suggested primer 2: | GCAGGGAGCTGAAGAACCAT |