Insertion junction: CLIP2.005000_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.005000
Chromosome:chromosome 6
Location:5250752
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g284200 LHCBM9 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACCCTGGTGTGAACAACGCCTTCGCCTTCGCCACCAAGTACACTCCCT
Internal bar code:TAGAATGAGATGTACTCGCAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3463
LEAP-Seq percent confirming:92.0
LEAP-Seq n confirming:92
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:100

Suggested primers for confirmation by PCR

Suggested primer 1:GGACACAACTGCAGGCTTTG
Suggested primer 2:TGGGGTAGGGACGGATGATT