Insertion junction: CLIP2.005066_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.005066
Chromosome:chromosome 3
Location:5590176
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g185550 SEBP1,SBP1 (1 of 2) 3.1.3.37 - Sedoheptulose-bisphosphatase / Sedoheptulose-1,7-bisphosphatase; Sedoheptulose-1%252C7-bisphosphatase, chloroplastic 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCGCGCCGAGTTTACGCACCCGAATCGCGGCACCCTCGGCCACTCCGT
Internal bar code:CTCTTGGCGGTAGGAGGCGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:961
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTTTGGATTTGGCGCT
Suggested primer 2:GGCATCCGAGGCTCTAATCC