| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005068 |
| Chromosome: | chromosome 12 |
| Location: | 5995437 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534250 | 3'UTR | ||
| Cre12.g534300 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTTTGAGGGTGGGGCGCACACCTGGGCGCATCAGGCGGCGGGCGGCG |
| Internal bar code: | GACGTCTCAAGAATTTTGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5215 |
| LEAP-Seq percent confirming: | 92.7273 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGTAGCAGCAGCAGGAG |
| Suggested primer 2: | TTGTTTGCTTGCTCGTGCTC |