Insertion junction: CLIP2.005077_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.005077
Chromosome:chromosome 17
Location:1825599
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g709350 SYP5 Qc-SNARE protein, Syn8/Syntaxin8-family; (1 of 1) K08503 - syntaxin of plants SYP5 (SYP5) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGAAGCTTTCATACAAGGAAATGCCATCAGGCAAGTCGCTTCACGCAG
Internal bar code:TGGGCTTGGCGAGCGGGGATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:969
LEAP-Seq percent confirming:90.4762
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GACGTAGCGTAGGAATGCGA
Suggested primer 2:TGCGGCTGTTTGAGACTGAT