Insertion junction: CLIP2.005115_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.005115
Chromosome:chromosome 7
Location:4263445
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre07.g341600 CGE1 (1 of 3) K03687 - molecular chaperone GrpE (GRPE); Chloroplast GrpE nucleotide release factor intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGAGCGCAACGGAAGGCCAAACCAGGAGATTGTTGGTGATGCCCTGG
Internal bar code:GAAATCTGTATTCGCTGGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:445
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAAGAATCCCTGTCCCGC
Suggested primer 2:TGCGCTTATCCCGTGTCTTT