Gene: Cre07.g341600

Overview

Common name:CGE1
Defline:(1 of 3) K03687 - molecular chaperone GrpE (GRPE); Chloroplast GrpE nucleotide release factor
Description:Chloroplast GrpE homolog, nucleotide release factor, co-chaperone of HSP70B, chloroplast-targeted; first intron is alternatively spliced : isoform CGE1b is more prominent at higher temperatures (>30 C) than CGE1a (which lacks a VQ dipeptide at position 4 of mature protein and prevails at lower temperatures); the protein is mostly localized to chloroplast stroma; participates with HSP70B in assembly/disassembly of VIPP1 oligomers.
Synonyms:g7956.t2,Cre07.g341600.t1.1,CGE1
Chromosome:chromosome 7

Protein Localization

A plasmid for expressing Cre07.g341600 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre07.g341600-Venus-3xFLAG

Imaged strain: CSI_LW01H12

Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.

Classification(s):

chloroplast/homogeneous/not pyrenoid depleted

cytosol/cytosol (weak signal)

If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.

VenusChlorophyllBright fieldMerged

Protein-Protein Interactions

We did not determine any protein-protein interactions for this gene.

Mutant Strains

Insertion Junction Mutant Strain Insertion Junction Feature Confidence (%) Genome Version
CLIP2.002722_3 CLIP2.002722 3'UTR 80 6.1
CLIP2.004275_1 CLIP2.004275 3'UTR 80 6.1
CLIP2.005115_1 CLIP2.005115 intron 63 6.1
CLIP2.005117_1 CLIP2.005117 intron 80 6.1
CLIP2.005117_2 CLIP2.005117 intron 80 6.1
LMJ.RY0402.048984_1 LMJ.RY0402.048984 3'UTR 95 5.5
LMJ.RY0402.048984_2 LMJ.RY0402.048984 3'UTR 95 5.5
LMJ.RY0402.163462_1 LMJ.RY0402.163462 3'UTR 58 5.5
LMJ.SG0182.016180_1 LMJ.SG0182.016180 MULTIPLE_SPLICE_VARIANTS 75 5.3

Gene Annotations

PFAM:PF01025
PANTHER: PTHR21237 PTHR21237:SF4
KEGG_ec:
KEGG_orthology:K03687
KOG:KOG3003
Gene ontology terms::GO:0000774, GO:0006457, GO:0042803, GO:0051087
Best arabidopsis TAIR name:AT5G17710
Best arabidopsis TAIR symbol:EMB1241
Best arabidopsis TAIR defline:Co-chaperone GrpE family protein
Phytozome Locus Page

Phenotypes

Our phenotype screens did not find statistically significant results for this gene.