| Insertion cassette: | pMJ013b |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.SG0182.016180 |
| Chromosome: | chromosome 7 |
| Location: | 4280717 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341600 | CGE1 | (1 of 3) K03687 - molecular chaperone GrpE (GRPE); Chloroplast GrpE nucleotide release factor | MULTIPLE_SPLICE_VARIANTS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATGACTGCAAGAAGGCCTG |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 535 |
| LEAP-Seq percent confirming: | 98.8439 |
| LEAP-Seq n confirming: | 171 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCAACACAGCATTGCCTC |
| Suggested primer 2: | AACAAAACTTGGCACGAACC |