| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048984 |
| Chromosome: | chromosome 7 |
| Location: | 4281159 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341600 | CGE1 | (1 of 3) K03687 - molecular chaperone GrpE (GRPE); Chloroplast GrpE nucleotide release factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTCGCTGATTGTGCTGTTGTCTGGAGCG |
| Internal bar code: | TAGGCCGGCGGGTAAAAGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 999 |
| LEAP-Seq percent confirming: | 98.2143 |
| LEAP-Seq n confirming: | 275 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATTGACTGCTCTCGGTGA |
| Suggested primer 2: | GGAATGGTTACGACAGGGTG |