| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005156 |
| Chromosome: | chromosome 16 |
| Location: | 1278563 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651400 | (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAAATGATGACGGCCTGACCATCCACCCGCCATCCGGAACGCGCACCA |
| Internal bar code: | GTGAGCTCCAGGTCTGTTGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2593 |
| LEAP-Seq percent confirming: | 82.8571 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAACCAGCATGCATTCGCC |
| Suggested primer 2: | CGACCCCTCCAGGATCATTG |