| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.005195 |
| Chromosome: | chromosome 12 |
| Location: | 2200937 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509000 | MAPK3 | Mitogen-Activated Protein Kinase 3; (1 of 3) K04371 - mitogen-activated protein kinase 1/3 (MAPK1_3) | 3'UTR |
| Cre12.g509050 | PPL1,PSBP3 | PsbP-like protein of thylakoid lumen; (1 of 1) PTHR31407:SF4 - PSBP-LIKE PROTEIN 1, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATTAGGCGCCGGATTATGGAGCTGACTGGCAACGCCGCTTGCGGTGTC |
| Internal bar code: | CATACAGGTCCAGAATTACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1760 |
| LEAP-Seq percent confirming: | 74.2857 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGTAGTGGATACGGACC |
| Suggested primer 2: | GTGGCTTAGGAGTCGACGAG |