| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.005211 |
| Chromosome: | chromosome 1 |
| Location: | 357586 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002150 | (1 of 5) IPR023220 - Type IV secretion system, VirB5-domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCTAATCAAAAGACAGCGCTTAGACCAAATACCGTATCACGTACGCA |
| Internal bar code: | AACTATTCCACACGATAGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1386 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGATTGCGTCGTAGTAGG |
| Suggested primer 2: | TTCCTTATGCCAAGCCCCTG |