Insertion junction: CLIP2.005236_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.005236
Chromosome:chromosome 6
Location:5369727
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g285250 LHCBM6 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chloropyll a/b binding protein of LHCII type I, chloroplast precursor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGGGCGGCAGCCTAAGGACACATGCGAAAGGGGGCAAGGAAAGCTTC
Internal bar code:TGCGAAATGGGGCGGAGCTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1124
LEAP-Seq percent confirming:37.7778
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:AACGCATTTGCAGTTGGGTC
Suggested primer 2:ACGCCGCTTGCATGATTTAC