| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005237 |
| Chromosome: | chromosome 16 |
| Location: | 2758224 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g662150 | CPLD51,CCB1 | conserved protein required for cyt b6 assembly; (1 of 1) PF12046 - Protein of unknown function (DUF3529) (DUF3529) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTCATGGGTTGCTCGTCGGCTCGTAACTGCGTAAGACCTGGACAGAGG |
| Internal bar code: | CATGAATGGTTACTGGTGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1635 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTGAGTTTGAAGTGCAC |
| Suggested primer 2: | GACAAGGAGGAGATCGAGCG |