| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005270 |
| Chromosome: | chromosome 2 |
| Location: | 1287579 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082300 | SUR6 | (1 of 1) PF04935 - Surfeit locus protein 6 (SURF6); Surfeit 6-like protein | 3'UTR |
| Cre02.g082350 | CUTA1,CUT1 | (1 of 1) K03926 - periplasmic divalent cation tolerance protein (cutA); Copper-binding protein CutA | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCCATGATGAAGGGGCTCATGTCAAATTTGTTGTGGTGTGCTAGGCC |
| Internal bar code: | TTACCGATTAGTCCTGGTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4116 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGGGCTGAGTTACGAGC |
| Suggested primer 2: | CAGGATTGACGGTTGCTCCT |