| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005481 |
| Chromosome: | chromosome 7 |
| Location: | 4634481 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344550 | (1 of 3) 1.1.1.95 - Phosphoglycerate dehydrogenase / Phosphoglyceric acid dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGAACATGTTACTAGGTGCCATCACTCCACCTCCTCTTCCATTATGAC |
| Internal bar code: | GTTGCAGTTTTCATATCAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5750 |
| LEAP-Seq percent confirming: | 95.4955 |
| LEAP-Seq n confirming: | 106 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 111 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACCTGGGGGAAGTTGAC |
| Suggested primer 2: | ACCTGCTCATGCTGCTTCTT |