Insertion junction: CLIP2.005484_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.005484
Chromosome:chromosome 8
Location:1649636
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g365900 LHCSR1,LI818 (1 of 3) PTHR21649//PTHR21649:SF15 - CHLOROPHYLL A/B BINDING PROTEIN // SUBFAMILY NOT NAMED; Stress-related chlorophyll a/b binding protein 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGCCTGCCACTAAACAAACTCGTGCCTGACTGTCGCGCCATCGCTTCC
Internal bar code:CGCAGAGAGACGGGTCTCCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1393
LEAP-Seq percent confirming:44.4444
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CCAACAGCGCGTGTGTAAAA
Suggested primer 2:CTCCCCACCCAACAGACTTC