| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005497 |
| Chromosome: | chromosome 12 |
| Location: | 5995619 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534250 | 3'UTR | ||
| Cre12.g534300 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGAATCACAATCTGCCAGTTCTGTAATCACACGAACGCCAGCCCAGAG |
| Internal bar code: | CATATCTTAGAGTGCCGTAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3530 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGTAGCAGCAGCAGGAG |
| Suggested primer 2: | TTGTTTGCTTGCTCGTGCTC |