Insertion junction: CLIP2.005575_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.005575
Chromosome:chromosome 7
Location:4620680
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g344400 (1 of 3) 1.1.1.95 - Phosphoglycerate dehydrogenase / Phosphoglyceric acid dehydrogenase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCTGACCCTTCACTTGCGCCTTGCGGCTACTGTTTACCACGGTGTGAC
Internal bar code:CAATTGGTCAAGAACGTGAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2784
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGGGTTTGCGATCGAAGT
Suggested primer 2:CCCTGCCATCATCTCGTCTC