| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005582 |
| Chromosome: | chromosome 12 |
| Location: | 2109938 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g510034 | (1 of 8) PF13432 - Tetratricopeptide repeat (TPR_16) | 3'UTR | |
| Cre12.g510050 | CTH1,CHL27B,CTH1B,CTH1A | Copper target homolog 1, chloroplast precursor%252C functional variant; (1 of 2) 1.14.13.81 - Magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase / Mg-protoporphyrin IX monomethyl ester oxidative cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGCATTGCGTTGTGCATACGCCGCGGCTTCGTACCTTTGCCTAACCT |
| Internal bar code: | TGGCACCTACGCAGAGCAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5728 |
| LEAP-Seq percent confirming: | 94.0476 |
| LEAP-Seq n confirming: | 158 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 168 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTATGCGCAGACCTGGAG |
| Suggested primer 2: | CTGATAGTGGCGCAAATGGC |