Insertion junction: CLIP2.005653_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.005653
Chromosome:chromosome 11
Location:2654863
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095077 (1 of 1) PF00571//PF04134 - CBS domain (CBS) // Protein of unknown function, DUF393 (DUF393) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCGGGCTGTGGGGACGGCGCGCGAGCATCGAACGGTCGCGGTACCTT
Internal bar code:CAACGGCTCAGTTTGAGTGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1290
LEAP-Seq percent confirming:53.3333
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AGGATACCCTGCGACTCCTT
Suggested primer 2:CAGTCCCATCACACTCCCAC