| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005674 |
| Chromosome: | chromosome 12 |
| Location: | 6596849 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538650 | UPM3,SUMT3,COBA,HEM4 | S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase; (1 of 1) 2.1.1.107 - Uroporphyrinogen-III C-methyltransferase / Uroporphyrinogen-III methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGCGTGGGCAGCCCCCGGAACGCCGCCTGCGTTCAGTGTGCGAGCAG |
| Internal bar code: | CGTACGATCCGTCGAGGGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 9.52381 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCAGGGCAAACAAGTGGT |
| Suggested primer 2: | CCCTCCTTATAACCGACCGC |