| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005731 |
| Chromosome: | chromosome 2 |
| Location: | 6229548 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387000 | CGL34 | (1 of 4) PF11891 - Domain of unknown function (DUF3411) (DUF3411); Conserved in the Green Lineage | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAAGGAAGCGCCCACCAGCGCAGCGACCTGCTGACCACGCAATCTTGC |
| Internal bar code: | GTATTTTCAAGGGGTAGGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4768 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCCCACGAATGCCTACA |
| Suggested primer 2: | GCGGCAAACGTGAGACTTTT |