| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.005788 |
| Chromosome: | chromosome 5 |
| Location: | 2183670 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237400 | DAE1 | (1 of 1) 5.1.1.7 - Diaminopimelate epimerase; Diaminopimelate epimerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCCACAAGCAGCAGCCTTACGCCCGGAGCTGGCCGCTGAAGACCAGC |
| Internal bar code: | TGAAGCCGAACAATAGTGTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5310 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 86 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATTGTTTCCGGCGAGCCT |
| Suggested primer 2: | GTCCCATTGTCCTCACAGCA |