Insertion junction: CLIP2.005828_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.005828
Chromosome:chromosome 2
Location:6798919
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388060 VDR1 violaxanthin de-epoxidase related, chloroplast; (1 of 1) 1.10.99.3 - Transferred entry: 1.23.5.1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGCCGCGGCCGGGGCTGCTGCCGGAGCCGGCAGCAGCAGCGGCGGCGG
Internal bar code:TTATATCGGCGGTAACTGGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:4304
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGCACCGATGTCTCAGAC
Suggested primer 2:CAGACCCTGAACACGTCACA