| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005835 |
| Chromosome: | chromosome 12 |
| Location: | 9723559 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554103 | CGL74 | (1 of 1) PTHR26312//PTHR26312:SF0 - FAMILY NOT NAMED // TETRATRICOPEPTIDE REPEAT PROTEIN 5; conserved expressed TPR repeat protein | 3'UTR |
| Cre12.g554528 | PPR10 | PentatricoPeptide Repeat protein 10; (1 of 2) PF01535//PF13812 - PPR repeat (PPR) // Pentatricopeptide repeat domain (PPR_3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCGAGAATGTGAGTTCGCGGATATCAGAGCCAATCCGCGCAGCAGGT |
| Internal bar code: | TGTCGCCAGAAACGACAGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 532 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAACAGCAACAGCATTGA |
| Suggested primer 2: | GGTGGTGGTGTAGAGGTGTG |