| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005847 |
| Chromosome: | chromosome 17 |
| Location: | 3737842 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726350 | (1 of 1) IPR000104//IPR002794 - Antifreeze protein, type I // Protein of unknown function DUF92, TMEM19 | 5'UTR | |
| Cre17.g726400 | (1 of 6) K04710 - ceramide synthetase (CERS) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGGGACGTTGACAATGAAAACGTCCTTTGATGGAGGTCAGCGTGCCGA |
| Internal bar code: | ATATTACGCGTTGGCAGAAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5084 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCACGGATATGACCCTTA |
| Suggested primer 2: | TGCTCACCTGTGCGATTTCT |