Insertion junction: CLIP2.005859_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.005859
Chromosome:chromosome 3
Location:5590776
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g185550 SEBP1,SBP1 (1 of 2) 3.1.3.37 - Sedoheptulose-bisphosphatase / Sedoheptulose-1,7-bisphosphatase; Sedoheptulose-1%252C7-bisphosphatase, chloroplastic intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCAAACACACATGCCTTGGACCCTGTCGGTGAGCGCTCACGCCACTCC
Internal bar code:CTTCTGTATTACTATAAGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:535
LEAP-Seq percent confirming:28.5714
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCTTGTCATCAACGTCCA
Suggested primer 2:AGGCTCACACAAACACCACA