Insertion junction: CLIP2.005859_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.005859
Chromosome:chromosome 3
Location:5590778
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g185550 SEBP1,SBP1 (1 of 2) 3.1.3.37 - Sedoheptulose-bisphosphatase / Sedoheptulose-1,7-bisphosphatase; Sedoheptulose-1%252C7-bisphosphatase, chloroplastic intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTGGCGCTAACCATTTTGACCGCATCACACTCACGCTCGCAGATCGAG
Internal bar code:CTTCTGTATTACTATAAGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3515
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:55
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCTCACACAAACACCACA
Suggested primer 2:TCGCTTGTCATCAACGTCCA