| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.005884 |
| Chromosome: | chromosome 5 |
| Location: | 2183936 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237400 | DAE1 | (1 of 1) 5.1.1.7 - Diaminopimelate epimerase; Diaminopimelate epimerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTATCAGTGCCCCCACTCCCGAAATCCGCACGCATCACGTGGGCTCT |
| Internal bar code: | TATAATAGTGAGCTCCAGTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2011 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTTGCCCGCAAGTATGT |
| Suggested primer 2: | CTCACAAGCACCACAAACGG |