Insertion junction: CLIP2.005904_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.005904
Chromosome:chromosome 7
Location:4620607
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre07.g344400 (1 of 3) 1.1.1.95 - Phosphoglycerate dehydrogenase / Phosphoglyceric acid dehydrogenase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAAGTCACTCTTACACTGTAATTGGAAGGGTAGCTGGGGGCCTATGTGC
Internal bar code:CTACACACGAATTATTCACCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:987
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTGCCATCATCTCGTCTC
Suggested primer 2:ACAGGGTTTGCGATCGAAGT