| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005979 |
| Chromosome: | chromosome 12 |
| Location: | 4358316 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g519300 | TEF9 | (1 of 2) PF13301 - Protein of unknown function (DUF4079) (DUF4079); conserved expressed protein, possible chloroplast localization | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCCAACACCCATCCCATTTCCCCGCGCCCCTCCCCGCCCCGTCTCCT |
| Internal bar code: | GTGGTGATGTTCTTGGCCATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2203 |
| LEAP-Seq percent confirming: | 63.6364 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGACACACACGTCCACG |
| Suggested primer 2: | TCCATACATACGCTCGGCAC |