| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005990 |
| Chromosome: | chromosome 1 |
| Location: | 6061781 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042750 | ACH1 | (1 of 1) 4.2.1.3 - Aconitate hydratase / Citrate(isocitrate) hydro-lyase; Aconitate hydratase | 3'UTR |
| Cre01.g042800 | DVR1 | 3%252C8-Divinyl protochlorophyllide a 8-vinyl reductase, chloroplast precursor; (1 of 1) K19073 - divinyl chlorophyllide a 8-vinyl-reductase (DVR) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCAATAAAGTTGCTGCGCAAGCGGTGCTGGGACAGCATCCTCTCTCTC |
| Internal bar code: | GGTTGGGATCCGCTTACAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2350 |
| LEAP-Seq percent confirming: | 96.3415 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTTGAACGCCGACTGCAA |
| Suggested primer 2: | TAATTGGAGGGAGGAGCGGA |