Insertion junction: CLIP2.005995_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.005995
Chromosome:chromosome 4
Location:3791114
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g229550 SRR15 Scavenger receptor cysteine rich (SRCR) protein; (1 of 5) 1.4.3.13 - Protein-lysine 6-oxidase / Lysyl oxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCCGGACCGCAGTGCCCGCGCCCCGCACGATGAGAGATCCAAGATGCC
Internal bar code:TCTTTGGTAAGTGGTGTTGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3418
LEAP-Seq percent confirming:91.8919
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCTGCCTTCCCAAAACAG
Suggested primer 2:ATGTTAGGGCACAGCTGTCC