Insertion junction: CLIP2.006002_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.006002
Chromosome:chromosome 16
Location:875990
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g647950 (1 of 33) IPR015797 - NUDIX hydrolase domain-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTTGAAGCAAGAGTCAGTCGCCGCCCAGAAGCTTCTGAACCCATATGCA
Internal bar code:GTTAGATCAAGGAGACGGGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2516
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCAGTAGCAGGGGAATAG
Suggested primer 2:GTGGTCCGGCACTTTGTTTC