Insertion junction: CLIP2.006006_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.006006
Chromosome:chromosome 6
Location:1009422
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g256750 FAT1,TEH9 Acyl carrier protein thioesterase; (1 of 1) K10782 - fatty acyl-ACP thioesterase A (FATA) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTGACAGGCCCTCAGACAACACTGTTGCGCATGTACCCTCCCCCGCCC
Internal bar code:CGTGTTGAATAATTAGACGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:949
LEAP-Seq percent confirming:71.4286
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ATGACCAATACCACCGCGTT
Suggested primer 2:CCCCTACACCCTACTCAGCT