Insertion junction: CLIP2.006108_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.006108
Chromosome:chromosome 16
Location:2667210
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g661550 (1 of 1) PF06941 - 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C) (NT5C) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTCCCTGCTTGCGGCCCCTGCACATTTGGTTTTCGAACTGCACACATG
Internal bar code:AATCACATCACGACTACCTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:4080
LEAP-Seq percent confirming:90.625
LEAP-Seq n confirming:58
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:64

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCTGGGGTCCATGATTTG
Suggested primer 2:GCAGTCAATGTTGTGTGCGT