| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006131 |
| Chromosome: | chromosome 4 |
| Location: | 680739 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217951 | LCI33 | Low-CO2-inducible protein; (1 of 9) IPR011011//IPR013083 - Zinc finger, FYVE/PHD-type // Zinc finger, RING/FYVE/PHD-type | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGTGCTTTTTCGTTCCCTAATGGCCTAATCCCCACACTTGCGTGCCG |
| Internal bar code: | TCTGTGCATTCATTTGGAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1200 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGCTCATCGACCCAGTG |
| Suggested primer 2: | CACCCAGCGTGAAGTTGTTG |