| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006138 |
| Chromosome: | chromosome 2 |
| Location: | 5103969 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g111450 | TEF4 | (1 of 25) PF00581 - Rhodanese-like domain (Rhodanese); Rhodanese-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCCGAAGTGCTGAATGGTATGCTCACTGGCGCATTGCAGGCAAGCCCA |
| Internal bar code: | AATGAGTCAGTGACATTTGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3663 |
| LEAP-Seq percent confirming: | 95.8763 |
| LEAP-Seq n confirming: | 93 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTTCAACCCACCCCTCC |
| Suggested primer 2: | CGGTGACTGCAGTCTCAGTT |