| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.006188 |
| Chromosome: | chromosome 3 |
| Location: | 4689959 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177350 | (1 of 4) PF11891 - Domain of unknown function (DUF3411) (DUF3411) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCTGTGTGCTCTGTTGGTAGATTCTTTTAACAATCCAACGGATGCTG |
| Internal bar code: | TGGGTATGTCGTAGCAGCTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1183 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACTGGACTTCGCGGTGAT |
| Suggested primer 2: | GCGGCGATGGATATTGAGGA |