Insertion junction: CLIP2.006220_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.006220
Chromosome:chromosome 12
Location:6032239
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g534700 FKB19 (1 of 1) PTHR10516:SF179 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP19, CHLOROPLASTIC; peptidyl-prolyl cis-trans isomerase, FKBP-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGGCTGCAGCGTGCGGGCGCCCGGGCATCGACGCGGCGATGCCAAACT
Internal bar code:TAGTAGAAACATCCCTGGGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1009
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGGCTCGAACTTGGACAG
Suggested primer 2:GGAGGTTGCATCGCGAATTC