| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006349 |
| Chromosome: | chromosome 9 |
| Location: | 2274349 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394750 | PRPS1 | (1 of 2) PTHR10724 - S1 RNA-BINDING DOMAIN-CONTAINING PROTEIN 1; Chloroplast Ribosomal Protein S1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAACACCCCATCAGGGTACCAAAATTTGGAGGCCCAAGAACGGAGCGC |
| Internal bar code: | CAAGCGTCATTTGGCCGGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4037 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 98 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 98 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATATGGCGGCAGGACAA |
| Suggested primer 2: | CGCCAGTATCTACCGTGCTT |