Insertion junction: CLIP2.006402_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.006402
Chromosome:chromosome 12
Location:328772
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g495951 CYN8,CYN20C,CYN20-3,ROC4 (1 of 4) K03768 - peptidyl-prolyl cis-trans isomerase B (cyclophilin B) [EC:5.2.1.8] (PPIB, ppiB); Cyclophilin 20-3 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATCACCCTCAGCTGCGTACACCGCTACTACGGTACTGCCGGGGCCGCT
Internal bar code:GCTGTCAATGTCGATGTTTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1829
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CACTCGTGACCGATACCTCG
Suggested primer 2:CCAGTTCAACCTTTCACGCG